复方中药对肉鸡腹水综合征肺组织NO、ET-1的作用及机制

doi:10.11843/j.issn.0366-6964.2015.06.023

Abstract

Abstract:

The objective of this study was to investigate the changes of the contents of nitric oxide (NO) and endothelin-1 (ET-1)，and the related items in lung of broilers with ascites syndrome (AS) and the prevention and treatment of the compound recipe of the traditional Chinese medicine on AS.A total of 316 14-day-old Ross broilers were randomly divided into normal group (N，bred with a standard diet at room temperature)，control group (C，bred with high-energy diet derived from standard diet added with 3% lard and 4% fish meal and 0.12% Na⁺ in drinking water at 9-11 ℃ to induce AS)，compound recipe of the traditional Chinese medicine groups (T1，T2，T3，bred in the same way as group C except a dose of 2，1，0.5 mL•kg^-1 bw of the traditional Chinese medicine everyday added in drinking water，respectively)，and L-arginine group (L，bred in the same way as group C except 1% L-arginine added in diet).The results showed that the broilers in group C presented the typical clinical symptoms of AS at 35 and 45 days of age.Compared with group N，the incidence of AS，ascites heart index (AHI)，the contents of NO and ET-1，iNOS activity and the relative expression amount of ETAR mRNA in lung of broilers at 35 and 45 days of age in group C were increased significantly (P<0.01 or P<0.05)，and cNOS activity and the ratio of NO to ET-1 were decreased significantly (P<0.01) in group C.The clinical symptoms and items mentioned above were all improved in groups of L，T1 and T2 compared with group C (P<0.01 or P<0.05).The study showed that the increases of NO and ET-1 content，ETAR mRNA relative expression amount and iNOS activity and the decrease of cNOS activity in lung of broiler induced the unbalance of NO and ET-1 which participated in the pathogenesis of AS.The studied compound recipe of the traditional Chinese medicine with the role of tonifying qi-blood and activating qi to excrete water can prevent and treat AS of broiler effectively.

CLC Number:

S853.33

HAN Ye-chen，SU Xiao-fei，WANG Jun-wu，ZHANG Juan，ZHU Yu-bo，CHENG Jia，YUN Shi-yu，SUN Yao-gui，WANG Wen-kui，LI Hong-quan，DUAN Zhi-bian. The Effect and Mechanism of the Compound Recipe of the Traditional Chinese Medicine on NO，ET-1 in Lung of Broiler with Ascites Syndrome[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 1055-1062.

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The Effect and Mechanism of the Compound Recipe of the Traditional Chinese Medicine on NO，ET-1 in Lung of Broiler with Ascites Syndrome

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